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By A. Ian Smith

The volumes during this sequence comprise modern ideas major to a specific department of neuroscience. they're a useful reduction to the coed in addition to the skilled researcher not just in constructing protocols in neuroscience yet in disciplines the place learn is turning into heavily with regards to neuroscience. each one quantity of tools in Neurosciences includes an index, and every bankruptcy comprises references. Dr. Conn turned Editor-in-Chief of the sequence starting with quantity 15, so every one next quantity might be guest-edited via a professional in that categorical box. This additional strengthens the intensity of insurance in equipment in Neurosciences for college kids and researchers alike. accomplished protocols incorporated for:* Enzymes enthusiastic about the activation of bioactive peptidases and proteins* Prohormone/neuropeptide processing pathways* Enzymes focused on peptide metabolism* Posttranslational processing enzymes

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305, 45 (1992). S. Benjannet, N. Rondeau, L. Paquet, A. Boudreault, C. Lazure, M. Chr6tien, and N. G. Seidah, Biochem. J. 294, 735 (1993). A. Boudreault, N. Rondeau, N. G. Seidah, M. Chr6tien, and C. Lazure, J. Biol. Chem. (submitted for publication) (1994). M. Marcinkiewicz, R. Day, N. G. Seidah, and M. Chr6tien, Proc. Natl. Acad. Sci. A. 90, 4922 (1993). -H. Sch~ifer and Robert Day Introduction Great progress has been made in the molecular identification of the enzymes involved in posttranslational processing of neuropeptides.

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Film is exposed for 24 to 96 hr and then developed. For mapping studies well-resolved images are obtained with Amersham Hyperfilm/3-max (Arlington Heights, IL) and Du Pont Cronex 4 film (Wilmington, DE), which are of finer grain. Photographic prints can be made directly from the X-ray image. Liquid Emulsion Autoradiography For microscopic analysis, the hybridized sections are coated with nuclear emulsion (Kodak, Rochester, NY or Ilford, London, UK). All procedures are performed in a darkroom illuminated only with a safe light (Kodak Safelight No.

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