Download Neurology Volume 72(4) January 27, 2009 by American Academy of Neurology PDF

By American Academy of Neurology

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Extra resources for Neurology Volume 72(4) January 27, 2009

Sample text

The t-test was used to compare the mean age at onset. 9), aged 16 to 75 years. 8). Treatment consisted of surgery (72 biopsies, 64 partial and 112 complete removals), followed by radiotherapy (43), radio ϩ chemotherapy (149), chemotherapy alone (32), or supportive care (30). The frequencies of the TP53 codon 72 variants for both the control and GBM population are reported in the table. 22 for GBM). There was no difference between the two populations for both genotype and allele frequencies. The same result was obtained when considering separately female and male populations.

The PCR reaction, performed in 15 ␮L with 20 ng genomic DNA, 1x TaqMan universal PCR master mix, forward and reverse primers (450 nmol/L each), 200 nmol/L VIC-labeled probe, 200 nmol/L FAM-labeled probe, was performed as follows: 95°C for 15 minutes, 50 cycles of 92°C for 30 seconds, 60°C for 1 minute. Completed PCR plates were read on an Mx3000P sequence detector and analyzed using the Mx Pro Q-PCR Software (Stratagene). Screening for TP53 gene mutations. Tumor DNA was extracted from frozen tumors using the QIAmp DNA minikit, as described by the manufacturer (Qiagen, Venlo, LN).

In multivariate analysis, surgery (hazard ratio ϭ Neurology 72 January 27, 2009 333 Figure 1 Survival curves according to genotype Figure 2 Pro/Pro frequency according to the age at diagnosis (A) The cumulative incidence of glioblastoma multiforme (GBM) for each codon 72 variant is plotted as a function of age. (B) The relative ratios of (Arg/Arg ϩ Arg/Pro) group and Pro/Pro group for the healthy controls, GBM patients diagnosed at Ͼ45 years of age, GBM patients diagnosed Ͻ45 years of age, and the independent series of GBM patients diagnosed at Ͻ45 years of age.

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