Download Candida Species: Methods and Protocols by Richard Calderone, Ronald Cihlar PDF

By Richard Calderone, Ronald Cihlar

This quantity offers distinct dialogue of numerous very important concepts that researchers use to check fungal molecular biology and pathogenesis. Written for the Methods in Molecular Biology sequence, chapters comprise introductions to their respective subject matters, lists of the required fabrics and reagents, step by step, without difficulty reproducible laboratory protocols, and tips about troubleshooting and warding off identified pitfalls.

Authoritative and useful, Candida Species: equipment and Protocols aims to make sure winning leads to the extra research of this very important field.

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7. Centrifuge at 3220 × g for 10 min at 4 °C. 8. Remove the mesh filter from the tube and aspirate off the supernatant. 9. Centrifuge at 3220 × g for 10 min at 4 °C. Aspirate off all the remaining liquid (see Note 22). 10. Weigh the tube and determine the sample weight. 11. Resuspend the pellet in TRIzol (~1 ml TRIzol per 50–100 mg sample) and add to the pre-chilled homogenization vials (prepared in step 2). Rinse the sample tube with additional TRIzol and add to the homogenization vial; there should only be a small amount of air left at the top of the vial (see Note 23).

Kumamoto Abstract Gene expression profiling has become an important tool for determining gene functions, performing phenotypic analysis, and quantifying the differential expression of individual transcripts under various conditions. Candida albicans gene expression is highly responsive to environmental conditions and rapidly adapts to various niches within the host. Here, we describe a mouse model of gastrointestinal colonization with C. albicans, the measurement of colonization in fecal pellets, and the collection of samples for transcriptional profiling.

8. If instead of mating patches a continuous line is visible, this clone (or one of the five if it is a mixture of colonies) carries both markers and the hyperosmotic stress test will not work. 9. Growing patches should be visible after 3 days. The stronger the interaction is the earlier the patches will grow. To remove background growth, re-replicate the plate on the same media, or on media with higher osmotic stress. Protein Association Assay 41 References 1. Fields S, Song O (1989) A novel genetic system to detect protein-protein interactions.

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