Download Analytical Methods in Human Toxicology: Part 2 by Alan S. Curry PDF

By Alan S. Curry

This choice of 9 essays by way of prime experts considers the applying of the main lately built analytical suggestions and gear to specific poisons and medication. It describes using HPLC, fluorescence research, and radioimmunoassays in toxicology and indicates equipment particular to forensic drugs.

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Analytical Methods in Human Toxicology: Part 2

This selection of 9 essays through prime specialists considers the appliance of the main lately built analytical options and gear to specific poisons and medicine. It describes using HPLC, fluorescence research, and radioimmunoassays in toxicology and exhibits equipment particular to forensic drugs.

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The pump should ideally be capable of providing a constant solvent flow without pressure fluctuations (or pulsing). The majority of pumps which are commercially available are fitted with some form of pulse damping mechanism (usually a long length of capillary tube) and are quite satisfactory for general use with spectrophotometric detectors . Special high-speed , dual-head reciprocating piston pumps may be required for use with electrochemical detectors since these detectors are extremely sensitive to variations in solvent flow rates .

3 Capacity Factor The capacity factor k is the ratio of the amount of solute in the stationary phase to the amount of solute in the mobile phase for a given solute at any point in the column equilibrium. Under constant conditions k may be used as a parameter for sample identification in a similar way to a retention index in gas/ liquid chromatography: 47 The Use ofHigh Performance Liquid Chromatography k = amount of solute in stationary phase amount of solute in mobile phase = tRI - to , to where to is the retention time for an unretained peak (equivalent to the solvent front in gas/liquid chromatography).

2. 2A) solvent passes from the pump into port 1 and out of port 2 to the A B from pump sample Q:1/ . r'. '-- .... 2 The loop injection valve. When the valve is oriented in position A solvent from the pump enters the valve through port 1 and exits it through port 2 directly to the column While the sample is applied via port 5 into the loop connecting ports 6 and 3. After the injection loop is loaded with sample the centre of the injection valve is rotated to position B, so that solvent from the pump then washes the sample from the loop out through port 2 to the column .

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